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| Inglis,Peter W.; Ciampi,Ana Y.; Salomão,Antonieta N.; Costa,Tânia da S.A.; Azevedo,Vânia C.R.. |
| Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the microgravity-treated seedlings compared to terrestrially germinated controls. Suppression subtractive hybridization of cDNA and EST analysis were used to detect differential gene expression in the microgravity-treated seedlings in comparison to those initially grown in normal gravity (forward subtraction). Despite their return to, and growth in normal gravity, the subtracted library derived from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microgravity; Stress response; Germination; Suppression subtractive hybridization; Zebrawood. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100014 |
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| Tang,Yue Yi; Wang,Chuan Tang; Yang,Guan Pin; Feng,Tong; Gao,Hua Yuan; Wang,Xiu Zhen; Chi,Xiao Yuan; Xu,Ya Long; Wu,Qi; Chen,Dian Xu. |
| To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known... |
| Tipo: Journal article |
Palavras-chave: Chilling response; Peanut; Real-time quantitative PCR; Suppression subtractive hybridization. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005 |
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| Ren,Xueying; Zhang,Xuecheng; Sui,Zhenghong. |
| Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples,... |
| Tipo: Journal article |
Palavras-chave: Female gametophyte; Gracilaria/Gracilariopsis lemaneiformis; Suppression subtractive hybridization; Tetrasporophyte; Virtual Northern blot. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006 |
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| Huvet, Arnaud; Herpin, Amaury; Degremont, Lionel; Labreuche, Yannick; Samain, Jean-francois; Cunningham, Charles. |
| Summer mortality associated with juveniles of the oyster Crassostrea gigas is probably the result of a complex interaction between the host, pathogens and environmental factors. Genetic variability in the host appears to be a major determinant in its sensitivity to summer mortality. Previously, divergent selection criteria based on summer survival have been applied to produce oyster families with resistant and susceptible progeny. In this paper, we describe the use of suppression subtractive hybridization to generate 150 C gigas clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these clones was determined. In 28%, the inferred amino sequence was found to match the products of known genes, 14%... |
| Tipo: Text |
Palavras-chave: Suppression subtractive hybridization; Oysters; Differentially regulated genes; Bivalves; Bacterial challenge. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-651.pdf |
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